Step One: Find an unfiltered beer
For this post, I was lucky enough to acquire a
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Great article, I was always curious about how to get enough yeast from a can to pitch. I'm a little confused about the cell count estimation. This solid/liquid ratio would also depend on the slurry concentration right? So if you have 10% in 50 mL like in the picture you have X amount of yeast but if you have that same amount of yeast in 100 mL, you would predict haven't X/2 amount of yeast. Am I not understanding this correctly? I think a better calculation would be the volume of the settled solids since the yeast cell diameter must be known and so this calculation would be relatively simple. That or use a hemocytometer.
posted by DICKERBEER on 2/27/2015 at 08:20:01 AM
I have been doing this for a while. I think it would be interesting and useful if everyone posted their favorite yeast to harvest maybe start a database. I have harvested from Chimay Blue didn't turn out to bad.
posted by adriedel on 2/27/2015 at 08:22:08 AM
This is awesome! I started growing my own yeast bank from White Labs vials about a month ago. There are lots of techniques here I can incorporate into my process.
posted by brianj on 2/27/2015 at 08:45:22 AM
adriel, that's awesome! I too think it would be super useful to get a yeast-harvesting database going somewhere. Hmmmm.
DICKERBEER, thanks! As I understand it, the approximation is based off of the settled solids. So the ml of liquid doesn't matter as much because you're etimating based off of percentages. So 10% solids in a 50 ml wouldn't be 10% solids in a 100 ml.
Sorry if I'm not understanding the objection!
posted by Matt on 2/27/2015 at 08:46:07 AM
I dunno why I wrote haven't, I meant having. I wasn't understanding what the 40-60% purpose was(hadn't finished my coffee yet) but I understand. I guess my only point is that there should be a way of calculating #yeast cells without having to create a slurry of 40-60% yeast, although that would not really be difficult. Thanks!
posted by DICKERBEER on 2/27/2015 at 09:23:52 AM
The Mad Fermentation has a list of unpasteurized and unfiltered bottles, although more sour/wild and not clean - here: http://www.themadfermentationist.com/p/dreg-list.html
posted by mchrispen on 2/28/2015 at 01:18:04 PM
So im happy to say i have succesfully harvest yeast from one of my homebrews from back in march. thanks for the tutorial. i've passed step one. The yeast looks like its responding great. Now for step two.... should i decant that first 150ml and add the slurry to the next 300ml? Or just keep that first 150ml and add it to another 150ml to total ~300? My stir plate arrives via amazon tomorrow. im excited.
posted by themikelee on 5/20/2015 at 12:43:02 AM
Tags for this post: yeast, bottle, beer, can, culture, grow, cells